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Guangjin Pan, Ph.D.
Principle Investigator

Dr. Pan achieved his Ph.D of biology from Tsinghua University, Medical School in 2005. Before that, he received Master degree in Medical Science from Shandong Academy of Medical Science in 2002. He then joined the University of Wisconsin- Madison for his Post Doc training. In 2010, he was recruited to GIBH as the principle investigator to set up a human pluripotent cell research lab. Dr. Pan has engaged in the embryonic stem cell research for more than 10 years.



Personnel: 


Du Juan

Associate scientist

Dr. Liao Baojian

Associate scientist

Dr. Wu Hongling

RA

Cai Xiujuan

RA

Zhang Jian

RA

Huang Wenhao

RA

Shi Xi

RA

Chen Qianyu

RA

Lin Aiping

RA

Wu Pengfei

RA

Man Ning

Student

Su Zhenghui

Student

Huangke

Student

Shan Yongli

Student

Li Xirui

Student

Meng Minghu

Student

Zhang Tian

Student

Shang Bizhi

Student

Zhu Yanling

Student

Zhang Yanqi

Student

Liang Zechuan

Student

 

 


Research:


Human pluripotent stem cells, either derived from the early embryo(ES), or induced from somatic cells (iPS), can undergo indefinite self-renewal and differentiate into any cell type in the human body, thus hold great potential for clinical use in regenerative medicine. On the one hand, our research is trying to understand what mechanisms control human pluripotent cells to choose between maintaining self renewal and exiting pluripotency to undergo specific lineage differentiation. Using human pluripotent stem cell differentiation as a model, we would analyze how different biological processes such as transcription factors mediated expression profile, signaling pathway, epigenetic change involved in control the cell fate transition. On the other hand, our research is trying to use human induced pluripotent cells to model some human disease. Our goals include highly efficient generating safe patient’s iPS cells, in vitro correcting its genetic defects, differentiating it into functional somatic cells for clinical use, et al.

 

Publications:

 

 My major findings include: Identified the negative feed back regulation loop among transcription factors in ES cells; Dissected the structural basis of biological function of these factors; Mapped the epigenetic patterns across the whole genome in human ES cells; Developed an efficient approach to directly convert human urine cells into neural progenitor cells. These findings had been published in 30 peer-reviewed papers including those in high profile journals such as Cell Stem Cell, Nat Methods, JBC, FASEB J. The total citation is 1714 and a the most citation for one single paper that I was the first author is 344. I was supported by “The hundred talent program”,CAS, and the chief scientist of national 973 major research plans. I won the second class of “National Natural Science Awards”. Currently, my researches are focusing on translational study of stem cell technology with aims to optimize induction and differentiation conditions for generation of functional cells for future clinical applications, for example, neural cells and blood cells.

* corresponding author.

1.  Wang, L., Wang, L., Huang, W., Su, H., Xue, Y., et al, Pan, G*., and Pei, D*. Generation of integration-free neural progenitor cells from cells in human urine. Nature Methods 10, 84-89 (2013) IF: 23.565. (ESI highly cited papercites=41)

2.       Ma NLiao BZhang HWang LShan Y, et al, Pan, G*. Transcription activator-like effector nuclease (TALEN)-mediated gene correction in integration-free β-thalassemia induced pluripotent stem cells. Journal of Biological Chemistry 288,34671-346792013IF: 4.651. (cites=23)

3.       Xue, Y., Cai, X., Wang, L., Liao, B., Zhang, H., et al, Pan, G*. Generating a Non-Integrating Human Induced Pluripotent Stem Cell Bank from Urine-Derived Cells. PLoS ONE8, e70573(2013) IF: 3.73. (cites=15)

4.       Liu, J., Wang, L., Su, Z., Wu, W., Cai, X., Li, D., Hou, J., Pei, D., and Pan, G*. A reciprocal antagonism between miR-376c and TGF-b signaling regulates neural differentiation of human pluripotent stem cells. Faseb Journal 28, 4642-4656.

5.  Huang, K., Jia, J., Wu, C., Yao, M., Li, M., et al, Pan, G*., and Yao, H*.  Ribosomal RNA Gene Transcription Mediated by the Master Genome Regulator Protein CCCTC-binding Factor (CTCF) Is Negatively Regulated by the Condensin Complex. Journal of Biological Chemistry 288, 26067-26077(2013) IF: 4.651.

6.       Huang KLiu PLi XChen SWang L, et al, Pan, G*. Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response. SCIENCE CHINA-Life Sciences 57,162-1702014IF: 2.024.

7.       Liu, P., Chen, S., Li, X., Qin, L., Huang, K., et al, Pan, G*., Cai, J*., and Pei, D*. Low Immunogenicity of Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Derived from Less Immunogenic Somatic Cells.PLoS ONE 8, e69617(2013) IF: 3.73.

8.  Pan, G., and Pei, D*. Order from Chaos: Single Cell Reprogramming in Two Phases. Cell Stem Cell 11, 445-447 . IF: 25.315.

9.  Pan, G., Wang, Tao., Yao, H., Pei D.* Somatic cell reprogramming for regenerative medicine: SCNT vs. iPS cells. BIOESSAYS 34 472-476 . IF: 5.423.

10.  Wang, T., Chen, K., Zeng, X., Yang, J., Wu, Y., Shi, X., Qin, B., Zeng, L., Esteban, Miguel A., Pan, G., and Pei, D*.The Histone Demethylases Jhdm1a/1b Enhance Somatic Cell Reprogramming in a Vitamin-C-Dependent Manner. Cell Stem Cell 9 575-587 (2011) IF: 25.315.

11.  Kuan, P. F., Chung, D., Pan, G., Thomson, J. A., Stewart, R., and Keleş, S. A statistical framework for the analysis of ChIP-Seq data. Journal of the American Statistical Association 106, 891-903(2011) IF: 4.651.

12.   Yu, P., Pan, G., Yu, J., and Thomson, James A. FGF2 Sustains NANOG and Switches the Outcome of BMP4-Induced Human Embryonic Stem Cell Differentiation. Cell Stem Cell 8, 326-334(2011) IF: 25.315.

13.  Xu, N., Papagiannakopoulos, T., Pan, G., Thomson, J. A., and Kosik, K. S. MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells. Cell 137, 647-658(2009) IF: 31.957.

14.  Xu, R.-H., Sampsell-Barron, T. L., Gu, F., Root, S., Peck, R. M., Pan, G., Yu, J., Antosiewicz-Bourget, J., Tian, S., Stewart, R., and Thomson, J. A. NANOG Is a Direct Target of TGF[beta]/Activin-Mediated SMAD Signaling in Human ESCs. Cell Stem Cell 3, 196-206(2008) IF: 25.315.

15.  Pan, G., Tian, S., Nie, J., Yang, C., Ruotti, V., Wei, H., Jonsdottir, G. A., Stewart, R., and Thomson, J. A. Whole-Genome Analysis of Histone H3 Lysine 4 and Lysine 27 Methylation in Human Embryonic Stem Cells. Cell Stem Cell 1, 299-312(2007) IF: 25.315. (cites=334)

16.  Pan, G*., and Thomson, J. A. NANOG and transcriptional networks in embryonic stem cell pluripotency.Cell Research 17, 42-49(2007) IF: 10.526. (cites=195)

17.  Pan, G., Pei, D. Molecular mechanisms on embryonic stem cell pluripotency. Chinese Bulletin of Life Sciences 19, 372-377(2007)

18.  Lee, M.-H., Hook, B., Pan, G., Kershner, A. M., Merritt, C., Seydoux, G., Thomson, J. A., Wickens, M., and Kimble, J. Conserved regulation of MAP kinase expression by PUF RNA-binding proteins. PLoS Genetics 3,2540-2550, e233(2007) IF: 8.517.

19.   Li, J., Pan, G., Cui, K., Liu, Y., Xu, S., and Pei, D. A dominant-negative form of mouse SOX2 induces trophectoderm differentiation and progressive polyploidy in mouse embryonic stem cells. Journal of Biological Chemistry 282, 19481-19492(2007) IF: 4.651.

20.  Pan, G., Li, J., Zhou, Y., Zheng, H., and Pei, D. A negative feedback loop of transcription factors that controls stem cell pluripotency and self-renewal. FASEB J 20, 1730-1732(2006) IF: 5.704. (cites=114)

21.  Shi, W., Wang, H., Pan, G., Geng, Y., Guo, Y., and Pei, D. Regulation of the pluripotency marker Rex-1 by NANOG and SOX2. Journal of Biological Chemistry 281, 23319-23325(2006) IF: 4.651.

22  Pan, G., and Pei, D. The stem cell pluripotency factor NANOG activates transcription with two unusually potent subdomains at its C terminus. Journal of Biological Chemistry 280, 1401-1407(2005) IF: 4.651.

23.  Pan, G., Qin, B., Liu, N., Scholer, H. R., and Pei, D. Identification of a nuclear localization signal in OCT4 and generation of a dominant negative mutant by its ablation. Journal of Biological Chemistry 279, 37013-37020(2004) IF: 4.651.

24.  Pan, G., and Pei, D. Identification of two distinct transactivation domains in the pluripotency sustaining factor NANOG. Cell Research 13, 499-502(2003) IF: 10.526.

25.   Pan, G., Chang, Z. Y., Scholer, H. R., and Pei, D. Stem cell pluripotency and transcription factor OCT4. Cell Research 12, 321-329(2002) IF: 10.526. (cites=141)

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