1、In vivo pharmacokinetics

A、 Blood concentration-time curve: Rats and mice were used as the basic animal models to detect the changing trend of blood concentration over time, and the relevant parameters of drug dynamics in vivo were calculated by fitting, such as bioavailability (BA%), half-life (T1/2), area under the curve (AUC), apparent volume of distribution (Vd), clearance rate in vivo, etc. (Cl).

B、 Drug determination of brain tissue and cerebrospinal fluid: The analysis of drug determination of brain tissue and cerebrospinal fluid is crucial for drug research whose drug target is located in the brain, and it is the key to analyze whether drugs can break through the blood-brain barrier and enter the brain.

C、Tissue distribution of drugs: Usually using rats and mice to determine the concentration of drugs in different tissues and organs to understand the approximate distribution and accumulation of drugs in the body.

D、Excretion of drugs: Usually performed using rats and mice, the approximate excretion of drugs in the animal is determined by analyzing the amount of drugs in the urine and feces.

2、ADME/T evaluation in vitro

A、Microsomal metabolic stability: The liver microsomes of different species (human, rat, mouse, dog) are used as the reaction system to measure the enzyme metabolic stability of drugs under the reaction of liver microsomal P450 enzyme system, and calculate the enzyme metabolic half-life of drugs, so as to predict the length of metabolic half-life in vivo.

B、Determination of binding rate of plasma protein: The degree of binding between drugs and plasma proteins is determined by the human plasma protein reaction system in vitro, and the binding between drugs and plasma proteins in vivo is predicted, so as to further judge the transport and balanced distribution of drugs in the body.

3、Pharmacodynamic evaluation

A、Efficacy experiments related to inflammation, including: Acute toe swelling experiment in rats (therapeutic and preventive); Toe tingling experiment in rats (therapeutic and preventive), half effective dose and single dose detection of COX2 inhibitors, etc.

B、Tumor-related efficacy evaluation experiment: Different tumor cells from different sources were inoculated subcutaneously to establish and evaluate related tumor animal models, and on the basis of this animal model, the efficacy experiment evaluation of anti-tumor drugs in vivo was completed.

C、Establishment and evaluation of animal disease models, including tumor model, diabetes model, pain model, acute inflammation model, etc.

4、Toxicological study

A、Acute toxicity in rats and mice. The first step in a toxicity study is the test of one exposure or multiple exposures over a 24-hour period. To observe the acute toxicity of the tested substance, to provide acute toxicity data, to determine the mode of toxic action, toxic reaction, and to provide reference for the subacute and chronic toxicity tests of observation indicators and dose grouping.

B、Repeated toxicity tests in rats and mice (7-30 days). The toxic effects of continuous exposure to large doses of a compound. The main purpose of this study was to search for toxic manifestations, toxic organs, threshold and non-active dose, and to provide dosage reference for further experiments.

C、Other toxicity evaluation: local drug toxicity, sensitization test, irritation test, etc.

Platform Personnel and Contact Information:

Person in Charge: Jingfang Xiong, 18102808661, xiong_jingfang@gibh.ac.cn, MiaoqinShe, 13672425207 , she_miaoqin@gibh.ac.cn