Proteome characteristic support platform equipped with Easy-nLC1200-Orbitrap Fusion Lumos with ETD ultra-high resolution liquid-mass spectrometry system, the instrument is additionally equipped with ETD fragmentation mode and FAIMS pro separation device. The instrument is mainly applied to proteomics-related research, which can provide interactome, proteomics, protein post-translational modification proteomics, and cross-linked proteomics research, and so on.The platform provides integrated technical services for sample preparation, testing, and data analysis. Proteomics analysis platform, as the core professional technology platform of Guangzhou Institutes of Biomedicine and Health, China Academy of Sciences (GIBH), integrates GIBH's major advanced instruments and equipment related to protein research, and possesses outstanding technological advantages in protein-protein interaction analysis, proteomic expression differences analysis , and protein post-translational modification omics. The platform is committed to providing high-quality technical support and services for scientific research exploration and talent cultivation of GIBH, and is also open to domestic and foreign research institutions and enterprises to promote collaborative development and innovation breakthroughs in the field of proteomics.

Equipment Information
The Orbitrap Fusion Lumos Tribrid mass spectrometer is ThermoFisher's advanced quadrupole- ultra-high field orbitrap-linear ion trap combination mass spectrometer with multiple collision modes: collision-induced dissociation (CID) and high-energy collision (HCD) and electron transfer deconvolution (ETD) modes, and the combination of ETD and HCD produces a double fragmentation ion fragmentation mode (EThcD), the mass spectrometer is equipped with a Nano ultra-high-pressure liquid-phase separation system.

Main technical parameters
1. Resolution: 500,000 (m/z=200)
2. mass range: 50-2,000 m/z (standard mode); 50-4,000 m/z (high mass mode)
3. Multi-stage mass spectrometry: 1-10 stages; two modes: high-resolution multi-stage and low-resolution multi-stage can be executed.
4. Scanning speed: 18 Hz (orbitrap), 20 Hz (ion trap)
5. mass accuracy: external standard method < 3 ppm; internal standard method < 1 ppm

Main applications
Combined with Easy-nLC 1200 nano-scale ultra-high performance liquid chromatograph, it can be used to complete the identification of simple proteins, large-scale identification of the protein composition of complex biological systems, protein-protein interaction, protein post-translational modification site characterization, protein post-translational modification omics, protein molecular weight determination, and other analysis. The details are as follows:

(1)Conventional proteomics identification and relative quantification, including label-free quanlitation and quantification (LFQ) based on DDA and DIA;
(2)Relative quantitative analysis of labeled proteomics, including iTRAQ, TMT, SILAC isotopes label-based quantification;
(3)Absolute quantitative analysis of proteins or peptides, including parallel reaction monitoring quantitative method (PRM, need to provide markers);
(4)Protein post-translational modification profiling sites with quantitative mass spectrometry analysis, including ubiquitination, phosphorylation, acetylation, methylation, and glycosylation modifications;
(5)Chemical proteomics analysis;
(6)Qualitative protein analysis by in-gel digestion;
(7)Chemical cross-linked mass spectrometry analysis;
(8)Provide personalized services according to the needs of users.

Equipment Information
Name: Ultra-High Resolution Liquid Mass Spectrometry System
Manufacturer: ThermoFisher Scientific
Model: Easy-nLC1200-Orbitrap Fusion Lumos
Contact: Tang Xiaoxiao
Contact: tang_xiaoxiao@gibh.ac.cn