Construction and application of gene modified large animal models; Development and optimization of novel gene editing technology.

1.       Weikai Ge^#, Shixue Gou^#, Xiaozhu Zhao^#, Qin Jin^#, Zhenpeng Zhuang, Yu Zhao, Yanhui Liang, Zhen Ouyang, Xiaoyi Liu, Fangbing Chen, Hui Shi, Haizhao Yan, Han Wu*, Liangxue Lai*, Kepin Wang*, In vivo evaluation of guide-free Cas9-induced safety risks in a pig model, Signal Transduction and Targeted Therapy, 2024, 9:184.

2.       Hui Shi^#, Lei Li^#, Shuangshuang Mu, Shixue Gou, Xiaoyi Liu, Fangbing Chen, Menglong Chen, Qin Jin*, Liangxue Lai*, Kepin Wang*, Exonuclease editor promotes precision of gene editing in mammalian cells, BMC Biology, 2024, 22:119.

3.       Shuangshuang Mu^#, Huangyao Chen^#, Qianru Li^#, Shixue Gou^#, Xiaoyi Liu, Junwei Wang, Wei Zheng, Menglong Chen, Qin Jin*, Liangxue Lai*, Kepin Wang*, Hui Shi*, Enhancing prime editor flexibility with coiled-coil heterodimers, Genome Biology, 2024, 25:108.

4.       Weikai Ge^#, Xiaozhu Zhao^#, Shixue Gou^#, Qin Jin^#, Fangbing Chen, Zhen Ouyang, Chengdan Lai, Tao Cui, Baoyi Mai, Sijia Lu, Kexin Zhong, Yanhui Liang, Tao Chen, Han Wu, Nan Li, Yinghua Ye, Liangxue Lai*, Kepin Wang*, Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos, Molecular Therapy-Nucleic Acids, 2023, 34:102035.

5.       Qin Jin^#, Hui Shi^#, Xiaoyi Liu, Jialu Ju, Kepin Wang*, Liangxue Lai*, Genome editing pig models with elements for controllable gene expression, Animal Research and One Health, 2023, 1(2):242-258.

6.       Jingke Xie^#, Zhenpeng Zhuang^#, Shixue Gou^#, Quanjun Zhang, Xia Wang, Ting Lan, Meng Lian, Nan Li, Yanhui Liang, Zhen Ouyang, Yinghua Ye, Han Wu, Liangxue Lai*, Kepin Wang*, Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription, Nucleic Acids Research, 2023, 51(18):10075-10093.

7.       Qin Jin^#, Xiaoyi Liu^#, Zhenpeng Zhuang^#, Jiayuan Huang^#, Shixue Gou, Hui Shi, Yu Zhao, Zhen Ouyang, Zhaoming Liu, Lei Li, Junjie Mao, Weikai Ge, Fangbing Chen, Manya Yu, Yezhi Guan, Yinghua Ye, Chengcheng Tang, Ren Huang, Kepin Wang*, Liangxue Lai*, Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation, Genome Biology, 2023, 24:8.

8.       Xunlei Wang^#, Qin Jin^#, Wenwen Xiao^#, Puxian Fang, Liangxue Lai, Shaobo Xiao, Kepin Wang*, Liurong Fang*, Genome-wide CRISPR/Cas9 screen reveals a role for SLC35A1 in the adsorption of porcine deltacoronavirus, Journal of Virology, 2022, 96(24):e0162622.

9.       Qin Jin^#, Xiaoyu Yang, Shixue Gou, Xiaoyi Liu, Zhenpeng Zhuang, Yanhui Liang, Hui Shi, Jiayuan Huang, Han Wu, Yu Zhao, Zhen Ouyang, Quanjun Zhang, Zhaoming Liu, Fangbing Chen, Weikai Ge, Jingke Xie, Nan Li, Chengdan Lai, Xiaozhu Zhao, Jiaowei Wang, Meng Lian, Lei Li, Longquan Quan, Yinghua Ye, Liangxue Lai*, Kepin Wang*, Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression, Science China Life Sciences, 2022, 65(11):2269-2286.

10.     Yanhui Liang^#, Jingke Xie^#, Quanjun Zhang^#, Xiaomin Wang^#, Shixue Gou, Lihui Lin, Tao Chen, Weikai Ge, Zhenpeng Zhuang, Meng Lian, Fangbing Chen, Nan Li, Zhen Ouyang, Chengdan Lai, Xiaoyi Liu, Lei Li, Yinghua Ye, Han Wu, Kepin Wang*, Liangxue Lai*, AGBE: A dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns, Nucleic Acids Research, 2022, 50(9):5384-5399.

11.     Nan Li^#, Shixue Gou^#, Jiaowei Wang^#, Quanjun Zhang, Xingyun Huang, Jingke Xie, Li Li, Qin Jin, Zhen Ouyang, Fangbing Chen, Weikai Ge, Hui Shi, Yanhui Liang, Zhenpeng Zhuang, Xiaozhu Zhao, Meng Lian, Yinghua Ye, Longquan Quan, Han Wu*, Liangxue Lai*, Kepin Wang*, CRISPR/Cas9-mediated gene correction in newborn rabbits with hereditary tyrosinemia type I, Molecular Therapy, 2021, 29(3):1001-1015.

12.     Meng Lian^#, Fangbing Chen^#, Xingyun Huang^#, Xiaozhu Zhao, Shixue Gou, Nan Li, Qin Jin, Hui Shi, Yanhui Liang, Jingke Xie, Weikai Ge, Zhenpeng Zhuang, Jiaowei Wang, Yinghua Ye, Yi Yang, Kepin Wang*, Liangxue Lai*, Han Wu*, Improving Cpf1-mediated base editing system by combining dCas9/ dead sgRNA with human APOBEC3A variants, Journal of Genetics and Genomics, 2021, 48(1):92-95.

13.     Wenjing Li^#, Lei Shi^#, Zhenpeng Zhuang^#, Han Wu^#, Meng Lian, Yihui Chen, Lei Li, Weikai Ge, Qin Jin, Quanjun Zhang, Yu Zhao, Zhaoming Liu, Zhen Ouyang, Yinghua Ye, Yingying Li, Hai Wang, Yuan Liao, Longquan Quan, Lei Xiao, Liangxue Lai*, Guangxun Meng*, Kepin Wang*, Engineered pigs carrying a gain-of-function NLRP3 homozygous mutation can survive to adult and accurately recapitulate human systemic spontaneous inflammatory responses, Journal of Immunology, 2020, 205(9):2532-2544.

14.     Jingke Xie^#, Xingyun Huang^#, Xia Wang, Shixue Gou, Yanhui Liang, Fangbing Chen, Nan Li, Zhen Ouyang, Quanjun Zhang, Weikai Ge, Qin Jin, Hui Shi, Zhenpeng Zhuang, Xiaozhu Zhao, Meng Lian, Jiaowei Wang, Yinghua Ye, Longquan Quan, Han Wu, Kepin Wang*, Liangxue Lai*, ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems, BMC Biology, 2020, 18:131.

15.     Jingke Xie^#, Weikai Ge^#, Nan Li^#, Qishuai Liu^#, Fangbing Chen, Xiaoyu Yang, Xingyun Huang, Zhen Ouyang, Quanjun Zhang, Yu Zhao, Zhaoming Liu, Shixue Gou, Han Wu, Chengdan Lai, Nana Fan, Qin Jin, Hui Shi, Yanhui Liang, Ting Lan, Longquan Quan, Xiaoping Li, Kepin Wang*, Liangxue Lai*, Efficient base editing for multiple genes/loci in pigs using base editors, Nature Communications, 2019, 10:2852.

16.     Han Wu^#, Qishuai Liu^#, Hui Shi, Jingke Xie, Quanjun Zhang, Zhen Ouyang, Nan Li, Yi Yang, Zhaoming Liu, Yu Zhao, Chengdan Lai, Degong Ruan, Jiangyun Peng, Weikai Ge, Fangbing Chen, Nana Fan, Qin Jin, Yanhui Liang, Ting Lan, Xiaoyu Yang, Xiaoshan Wang, Zhiyong Lei, Pieter A. Doevendans, Joost P. G. Sluijter, Kepin Wang*, Xiaoping Li*, Liangxue Lai*, Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems, Cellular and Molecular Life Sciences, 2018, 75(19):3593-3607.

17.     Degong Ruan^#, Jiangyun Peng^#, Xiaoshan Wang^#, Zhen Ouyang^#, Yi Yang, Fangbing Chen, Weikai Ge, Han Wu, Zhaoming Liu, Yu Zhao, Bentian Zhao, Quanjun Zhang, Chengdan Lai, Nana Fan, Zhiwei Zhou, Qishuai Liu, Nan Li, Qin Jin, Hui Shi, Jingke Xie, Xiaoyu Yang, Hong Song, Kepin Wang*, Xiaoping Li*, Liangxue Lai*, Xist derepression in active X chromosome hinders pig somatic cell nuclear transfer, Stem Cell Reports, 2018, 10(2): 494-508.

18.     Kepin Wang^#, Qin Jin^#, Degong Ruan^#, Yi Yang, Qishuai Liu, Han Wu, Zhiwei Zhou, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Bentian Zhao, Quanjun Zhang, Jiangyun Peng, Chengdan Lai, Nana Fan, Yanhui Liang, Ting Lan, Nan Li, Xiaoshan Wang, Xinlu Wang, Yong Fan, Pieter A. Doevendans, Joost P.G. Sluijter, Pentao Liu, Xiaoping Li*, Liangxue Lai*, Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing, Genome Research, 2017, 27(12): 2061-2071.

19.     Yi Yang^#, Kepin Wang^#, Han Wu, Qin Jin, Degong Ruan, Zhen Ouyang, Bentian Zhao, Zhaoming Liu, Yu Zhao, Quanjun Zhang, Nana Fan, Qishuai Liu, Shimei Guo, Lei Bu, Yong Fan, Xiaofang Sun, Xiaoping Li*, Liangxue Lai*, Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering, Journal of Molecular Cell Biology, 2016, 8(2): 174-177.