PAN Guangjin--Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences

Faculty

PAN Guangjin

Title：Principle Investigator

Subject：

Email：pan_guangjin@gibh.ac.cn

Address：No.190 Kaiyuan Road, Guangzhou Science Park, Luogang District, Guangzhou 510530

Study/Work Experience

2010 – now	Guangzhou Institutes of Biomedicine and Health (CAS)/Principal Investigator ,Deputy Director General
2008 – 2010	University of Wisconsin-Madison/Assistant Scientist
2005 – 2008	University of Wisconsin-Madison/Post-doc Fellow
2002 – 2005	Tsinghua University/Doctoral student
1999 – 2002	Shandong Academy of Medical Science/ Postgraduate student
1994 – 1998	Shandong University/Bachelor's degree

Research Areas

The research in Pan’s laboratory focuses on three parts mainly: 1. To understand the mechanisms by which control the cell fate maintenance and transition of hPSCs. Using hPSCs as a model, the lab analyzes how different biological processes such as transcription factors mediated expression profile, signaling pathway, epigenetic change involved in the regulation of cell fate transition; 2. To generate functional neural stem cells (NSC) and hematopoietic stem/progenitor cells (HSC/HPC) from hPSCs. Trying to gain functional HPC and reveal the mechanisms involved in differentiation regulation through an integrated approach using molecular biology, cell biology, epigenetics, genomics, FACS, confocal microscopy and so on. For the hPSCs derived NSCs, after the NSCs are tested for their therapeutic potential in animal models, the final goal of this research is to translate the technology to the re-building of the injured or diseased human brain.

Academic Performance

2005.06 Outstanding PhD student, Tsinghua University

2012.12 Outstanding staff award, GIBH, CAS

2013. 12 National Natural Science Awards(Second Class)

2018. 01 Outstanding Scientific and Technological Achievement Award of Chinese Academy of Sciences (outstanding contributor)

2018. 12 National Natural Science Awards(Second Class)

2019. 03 Guangdong Science Awards(First Class)

2019. 12 Guangdong Province Special Program for Outstanding

Representative Papers

1. Guo, X., Zhao, K., Zhang, Y., Zhou, T., and Pan, G*. (2024) Generation of a PPM1A-deficient human induced pluripotent stem cell line using CRISPR-Cas9 technology. Stem Cell Research 77, 103420.

2. Li, F., Zhu, Y.*, Wang, T., Tang, J., Huang, Y., Gu, J., Mai, Y., Wang, M., Zhang, Z., Ning, J., Kang, B., Wang, J., Zhou, T., Cui, Y., and Pan, G*. (2024) Characterization of gene regulatory networks underlying key properties in human hematopoietic stem cell ontogeny. Cell Regeneration 13.

3. Gu, J#., Zhu, Y#., Lin, H., Huang, Y., Zhang, Y., Xing, Q., Kang, B., Zhang, Z., Wang, M., Zhou, T., Mai, Y., Chen, Q., Li, F., Hu, X., Wang, S., Peng, J., Guo, X., Long, B., Wang, J., Gao, M., Shan, Y., Cui, Y., and Pan, G*. (2024) Autophagy is essential for human myelopoiesis. Stem Cell Reports 19, 196-210.

4. Zhang, C., Shan, Y., Lin,H., Zhang,Y., Xing,Q., Zhu, J., Zhou, T., Lin, A., Chen, Q., Wang, J., and Pan, G*. (2024) HBO1 determines SMAD action in pluripotency and mesendoderm specification. Nucleic acids research

5. Kang, B., Xing, Q., Huang, Y., Lin, H., Peng, J., Zhang, Z., Wang, M., Guo, X., Hu, X., Wang, S., Wang, J., Gao, M., Zhu, Y*., and Pan, G*. (2024) Large-scale generation of IL-12 secreting macrophages from human pluripotent stem cells for cancer therapy. Molecular therapy. Methods & clinical development 32, 101204.

6. Zhang, J., Wang, T., Shi, R., Zhao, Y., Zhang, Y., Zhang, C., Xing, Q., Zhou, T., Shan, Y., Yao, H., Zhang, X., and Pan, G*. (2024) YTHDF1 facilitates PRC1-mediated H2AK119ub in human ES cells. Journal of Cellular Physiology 239, 152-165.

7. Wang, J., Xie, W., Li, Nan; Li, W., Zhang, Z., Fan, N., Ouyang, Z., Zhao, Y., Lai, C., Li, H., Chen, M., Quan, L., Li, Y., Jiang, Y., Jia, W., Fu, L., Mazid, M., Zhu, Y., Maxwell, P., Pan, G*., Esteban, M*., Dai, Z*., and Lai, L*. (2023) Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation. Cell stem cell 30, 1235.

8. Zhang, C., Lin, H., Zhang, Y., Xing, Q., Zhang, J., Zhang, D., Liu, Y., Chen, Q., Zhou, T., Wang, J., Shan, Y., and Pan, G*. (2023) BRPF1 bridges H3K4me3 and H3K23ac in human embryonic stem cells and is essential to pluripotency. Iscience 26.

9. Zhang, D., Zhou, M., Zhang, Y., Shan, Y*., and Pan, G*. (2022) Generation of an RNF1-deficient human pluripotent stem cell line using CRISPR/Cas9 technology. Stem Cell Research 62, 102809.

10. Zhang, J., Zhou, T., Shan, Y., and Pan, G*. (2022) Generation of RYBP FLAG-HA knock-in human embryonic stem cell line through CRISPR/Cas9-mediated homologous recombination. Stem Cell Research 62, 102803.

11. Zhu, Y#., Zhang, Z#., Fan, N#., Huang, K., Li, H., Gu, J., Zhang, Q., Ouyang Z., Zhang, T., Tang, J., Zhang, Y., Suo, Y., Lai, C., Wang, J., Wang, J., Shan, Y., Wang, M., Chen, Q., Zhou, T., Lai, L*., and Pan, G*. (2022) Generating functional cells through enhanced interspecies chimerism with human pluripotent stem cells. Stem Cell Reports 17, 1059-1069.

12. Kang, B#., Zhang, T#., Huang, K#., Wang, T., Li, Y., Mai, Y., Li, J., Dang, S., Zhang, Z., Huang, W., Wang, J., Gao, M., Wang, Y., and Pan, G*. (2022) GFI1 regulates chromatin state essential in human endothelial-to-haematopoietic transition. Cell Proliferation 55, e13244.

13. Long, B#., Shan, Y#., Sun, Y.#, Wang, T., Li X., Huang, K., Zhang, W., He, Y., Wen, R., Li, Y., Mai, Y., Feng, Y., Zhang, T., Kang, B., Zhang, C., Zhu, Y., Gu, J., Liu, J., Zhang, X*., and Pan, G*. (2022) Vitamin C promotes anti-leukemia of DZNep in acute myeloid leukemia. Biochimica et Biophysica ACTA-Molecular Basis of Disease 1868, 166357.

14. Wu, R#., Guo, Y#., Zhang, L#., Zheng, H., Chen, X., Li, M., Xing, Q., Huang, W., Su, Z., Zhang, D., Zhong, X., Pan, G*., and Hu, X*. (2022) Physical exercise promotes integration of grafted cells and functional recovery in an acute stroke rat model. Stem Cell Reports 17, 276-288.

15. Li, Y., Gao, J., Guan, Y., Wei, X., Li, X., Li, Y., Huang, Z., Liu, S., Li, G., Xu, P., Pan, G*., and Zhang, Y*. (2021) Novel rhino-like SHJH(hr) mice with thyroid dysfunction. Zoological Research 42, 734-738.

16. Wei, Y., Wang, T., Ma, L., Zhang, Y., Zhao, Y., Lye, K., Xiao, L., Chen, C., Wang, Z., Ma, Y., Zhou, X., Sun, F., Li, W., Dunk, C., Li, S., Nagy, A., Yu, Y., Pan, G*., Lye, S*., and Shan, Y*. (2021) Efficient derivation of human trophoblast stem cells from primed pluripotent stem cells. Science Advances 7, eabf4416.

17. Zhu, Y#., Wang, T#., Gu, J#., Huang, K., Zhang, T., Zhang, Z., Liu, H., Tang, J., Mai, Y., Zhang,Y., Li, Y., Feng, Y., Kang, B., Li, J., Shan, Y., Chen, Q., Zhang, J., Long, B., Wang, J., Gao, M., Zhang, D., Zhou, M., Zhong, X., Chen, J., Pei, D., Nie, J., Liu, B., Pan, G*. (2020) Characterization and generation of human definitive multipotent hematopoietic stem/progenitor cells. Cell Discovery 6, 89.

18. Sun, W#., Zhang, S#., Zhou, T., Shan, Y., Cao, F., Zhang, Y., Zhang, D., Xiong, Y., Mai, Y., Fan, K., Alan J. Davidson, Pan, G*., Zhang, X*.(2020) Human Urinal Cell Reprogramming: Synthetic 3D Peptide Hydrogels Enhance Induced Pluripotent Stem Cell Population Homogeneity. ACS BIOMATERIALS SCIENCE & ENGINEERING 6, 6263-6275.

19. Shan, Y#., Zhang, Y#., Zhao, Y#., Wang, T., Zhang, J., Yao, J., Ma, N., Liang, Z., Huang, W., Huang, K., Zhang, T., Su, Z., Chen, Q., Zhu, Y., Wu, C., Zhou, T., Sun, W., Wei, Y., Zhang, C., Li, C., Su, S., Liao, B., Zhong, M., Zhong, X., Nie, J.,Pei, D., Pan, G*. (2020) JMJD3 and UTX determine fidelity and lineage specification of human neural progenitor cells. Nature Communications 11, 382.

20. Zhang, T#., Huang, K#., Zhu, Y., Wang, T., Shan, Y., Long, B., Li, Y., Chen, Q., Wang, P., Zhao, S., Li, D., Wu, C., Kang, B., Gu, J., Mai, Y., Wang, Q., Li, J., Zhang, Y., Liang, Z., Guo, L., Wu, F., Su, S., Wang, J., Gao, M., Zhong, X., Liao, B., Chen, J., Zhang, X.,Shu, X., Pei, D., Nie, J., Pan, G*. (2019) Vitamin C-dependent lysine demethylase 6 (KDM6)-mediated demethylation promotes a chromatin state that supports the endothelial-to-hematopoietic transition. Journal of Biological Chemistry 294, 13657-13670.

21. Xing, Q., Lin, A., Su, Z., Liu, C., Huang, W., Guo, W., Pan, G., Guo, Y*., and Zhong, X*. (2019) Retrograde monosynaptic tracing through an engineered human embryonic stem cell line reveals synaptic inputs from host neurons to grafted cells. Cell Regeneration

22. Huang, K#., Zhu, Y#., Ma, Y., Zhao, B., Fan, N., Li, Y., Song, H., Chu, S., Ouyang, Z., Zhang, Q., Xing, Q., Lai, C., Li, N., Zhang, T., Gu, J., Kang, B., Shan, Y., Lai, K., Huang, W., Mai, Y., Wang, Q., Li, J., Lin, A., Zhang, Y., Zhong, X., Liao, B., Lai, L., Chen, J., Pei, D., Pan, G*. (2018) BMI1 enables interspecies chimerism with human pluripotent stem cells. Nature Communications 9, 4649.

23. Su, Z#., Zhang, Y., Liao, B., Zhong, X., Chen, X., Wang, H., Guo, Y., Shan, Y., Wang, L., Pan, G*. (2018) Antagonism between the transcription factors NANOG and OTX2 specifies rostral or caudal cell fate during neural patterning transition. Journal of Biological Chemistry 293, 4445-4455.

24. Kang, S#., Chen, X., Gong, S., Yu, P., Yau, S., Su, Z., Zhou, L., Yu, J., Pan, G*., Shi, L*. (2017) Characteristic analyses of a neural differentiation model from iPSC-derived neuron according to morphology, physiology, and global gene expression pattern. Scientific Reports 7, 12233.

25. Shan, Y#., Liang, Z#., Xing, Q., Zhang, T., Wang, B., Tian, S., Huang, W., Zhang, Y., Yao, J., Zhu, Y., Huang, K., Liu, Y., Wang, X., Chen, Q., Zhang, J., Shang, B., Li, S., Shi, X., Liao, B., Zhang, C., Lai, K., Zhong, X., Shu, X., Wang, J., Yao, H., Chen, J., Pei, D., Pan, G*. (2017) PRC2 specifies ectoderm lineages and maintains pluripotency in primed but not naïve ESCs. Nature Communications 8, 672.

26. Wang, W#., Zhu, Y#., Huang, K., Shan, Y., Du, J., Dong, X., Ma, P., Wu, P., Zhang, J., Huang, W., Zhang, T., Liao, B., Yao, D., Pan, G* and Liu, J*. (2017) Suppressing P16Ink4a and P14ARF pathways overcomes apoptosis in individualized human embryonic stem cells. The FASEB Journal 31, 1130-1140.

27. Huang, K#., Gao, J#., Du, J#., Ma, N., Zhu, Y., Wu, P., Zhang, T., Wang, W., Li, Y., Chen, Q., Hutchins, A., Yang, Z., Zhang,J., Shan, Y., Li, X., Liao, B., Liu, J., Wang, J., Liu, B., Pan, G*. (2016) Generation and analysis of GATA2w/eGFP 2 human ESCs reveal ITGB3/CD61 as a reliable marker for defining hemogenic endothelial cells during hematopoiesis. Stem Cell Reports 7, 854-868.

28. Wang, L., Li, X., Huang, W., Zhou, T., Wang, H., Lin, A., Hutchins, A. P., Su, Z., Chen, Q., Pei, D., and Pan, G*. (2016) TGFβ signaling regulates the choice between pluripotent and neural fates during reprogramming of human urine derived cells. Scientific Reports 6, 22484.

29. Li, D., Wang, L., Hou, J., Shen, Q., Chen, Q., Wang, X., Du, J., Cai, X., Shan, Y., Zhang, T., Zhou, T., Shi, X., Li, Y*., Zhang, H*., and Pan, G*. (2016) Optimized Approaches for Generation of Integration-free iPSCs from Human Urine-Derived Cells with Small Molecules and Autologous Feeder. Stem Cell Reports 6(5), 717-728.

30. Wang, H., Hu, L., Liu, C., Su, Z., Wang, L., Pan, G*., Guo, Y*., He, J*. (2016) 5-HT2 receptors mediate functional modulation of GABAa receptors and inhibitory synaptic transmissions in human iPS-derived neurons. Scientific Reports 6, 20033.

31. Zhou, P., Wu, F., Zhou, T., Cai, X., Zhang, S., Zhang, X., Li, Q., Li, Y., Zheng, Y., Wang, M., Lan, F., Pan, G*., Pei, D., Wei, S*. (2016) Simple and versatile synthetic polydopamine-based surface supports reprogramming of human somatic cells and long-term self-renewal of human pluripotent stem cells under defined conditions. Biomaterials 87, 1-17.

32. Huang, K., Du, J., Ma, N.,Liu, J, Wu, P., Dong, X., Meng,M., Wang, W., Chen, X., Shi, X., Chen, Q., Yang, Z., Chen, S., Zhang, J., Li, Y., Li, W.,Zheng, Y., Cai, J., Li, P., Sun, X., Wang, J., Pei, D., Pan, G*. (2015) GATA2(-/-) human ESCs undergo attenuated endothelial to hematopoietic transition and thereafter granulocyte commitment.Cell Regeneration 4(1):4.

33. Ma, N#., Shan, Y#., Liao, B#., Kong, G#., Wang, C., Huang, K., Zhang, H., Cai, X., Chen, S., Pei, D., Chen, N*., and Pan, G*. (2015) Factor-induced Reprogramming and Zinc Finger Nuclease-aided Gene Targeting Cause Different Genome Instability in β-Thalassemia Induced Pluripotent Stem Cells (iPSCs). Journal of Biological Chemistry 290, 12079-12089.

34. Huang, K#., Liu, P#., Li, X., Chen, S., Wang, L., Qin, L., Su, Z., Huang, W., Liu, J., Jia, B., Liu, J., Cai, J*., Pei, D*., Pan, G*. (2014) Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response. SCIENCE CHINA-Life Sciences 57,162-170.

35. Jia, B#., Chen, S#., Zhao, Z., Liu, P, Cai, J., Qin, D., Du, J., Wu, C., Chen, Q., Cai, X., Zhang, H., Yu, Y., Pei, D., Zhong, M*., and Pan, G*. (2014) Modeling of hemophilia A using patient-specific induced pluripotent stem cells derived from urine cells. Life Sciences 108, 22-29.

36. Liu, J., Wang, L., Su, Z., Wu, W., Cai, X., Li, D., Hou, J., Pei, D., and Pan, G*. (2014) A reciprocal antagonism between miR-376c and TGF-beta signaling regulates neural differentiation of human pluripotent stem cells. FASEB Journal 28, 4642-4656.

37. Wang, L., Wang, L., Huang, W., Su, H., Xue, Y., Su, Z., Liao, B., Wang, H., Bao, X., Qin, D., He, J., Wu, W., So, K. F., Pan, G*., and Pei, D*. (2013) Generation of integration-free neural progenitor cells from cells in human urine. Nature Methods 10, 84-89.

38. Ma, N#., Liao, B#., Zhang, H., Wang, L., Shan, Y., Xue, Y., Huang, K., Chen, S., Zhou, X., Chen, Y., Pei, D., and Pan, G*. (2013) Transcription Activator-like Effector Nuclease (TALEN)-mediated Gene Correction in Integration-free beta-Thalassemia Induced Pluripotent Stem Cells. Journal of Biological Chemistry 288, 34671-34679.

39. Xue, Y., Cai, X., Wang, L., Liao, B., Zhang, H., Shan, Y., Chen, Q., Zhou, T., Li, X., Hou, J., Chen, S., Luo, R., Qin, D., Pei, D., and Pan, G*. (2013) Generating a Non-Integrating Human Induced Pluripotent Stem Cell Bank from Urine-Derived Cells. PLoS ONE 8, e70573.

40. Liu, P#., Chen, S#., Li, X., Qin, L., Huang, K., Wang, L., Huang, W., Li, S., Jia, B., Zhong, M., Pan, G*., Cai, J*., and Pei, D*. (2013) Low Immunogenicity of Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Derived from Less Immunogenic Somatic Cells.PLoS ONE 8, e69617.

41. Huang, K#., Jia, J#., Wu, C., Yao, M., Li, M., Jin, J., Jiang, C., Cai, Y., Pei, D., Pan, G*., and Yao, H*. (2013) Ribosomal RNA Gene Transcription Mediated by the Master Genome Regulator Protein CCCTC-binding Factor (CTCF) Is Negatively Regulated by the Condensin Complex. Journal of Biological Chemistry 288, 26067-26077.

42. Pan, G., and Pei, D*. (2012) Order from Chaos: Single Cell Reprogramming in Two Phases. Cell Stem Cell 11, 445-447.

43. Pan, G., Wang, Tao., Yao, H., Pei D.* (2012) Somatic cell reprogramming for regenerative medicine: SCNT vs. iPS cells. BIOESSAYS 34 472-476.