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2007.12 – now |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences/ Principal Investigator |
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1998.10 – 2006.10 |
University of Missouri-Columbia. USA/Postdoctoral Fellow, Research assistant professor |
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1995.09 – 1998.10 |
Changchun University of Agriculture and Animal Sciences, Changchun, P. R. China/Postdoctoral Fellow |
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1992.09 – 1995.07 |
Northeast Agricultural University, China/ Ph.D |
As a principal investigator in Guangzhou Institute of Biomedicine and Health Chinese Academy of Sciences, Dr. Lai’s lab is focusing on genetic modification of large animals, Human and animal stem cells, and animal cloning.
Dr. Lai has been undertaking many national major scientific research programs, such as 973 program (National Program on Key Basic Research Projects), 863 Program (National High-tech R&D Program of China). He has published more than 200 peer review papers in the international journals including Science, Cell, Cell Stem Cell, and Nature Biotechnology. He was a winner of Best of What’s New Grand Award from Popular Science in the medical Technology category, 2002. His two achievements were listed as world top 100 science news story by Discover magazine. As of now, his team has produced more than 100 kinds of genetically modified large animals including pigs, dogs and rabbits with important applications in biomedicine and agriculture.
Recipient of the National Overseas Outstanding Youth Fund；
Winners of the talent introduction plan of the Chinese Academy of Sciences
State Council Government Special Allowance；
Guangdong Province's "Top 100 Heroes of Southern Guangdong"；
First prize of Guangdong Science and Technology Award (first author)；
First prize of Jilin Province Science and Technology Award (first author);
First prize of Guangzhou Science and Technology Award (first author);
Won the 2002 World's Latest Medical Technology Award;
Two achievements were selected as one of the world's top 100 technology news of the year by Discovery magazine in the United States.
1. Weikai Ge#, Shixue Gou#, Xiaozhu Zhao#, Qin Jin#, Zhenpeng Zhuang, Yu Zhao, Yanhui Liang, Zhen Ouyang, Xiaoyi Liu, Fangbing Chen, Hui Shi, Haizhao Yan, Han Wu*, Liangxue Lai*, Kepin Wang*, In vivo evaluation of guide-free Cas9-induced safety risks in a pig model, Signal Transduction and Targeted Therapy, 2024. Accepted.
2. Jiaowei Wang#, Wenguang Xie#, Nan Li#, Wenjuan Li#, Zhishuai Zhang#, Nana Fan, Zhen Ouyang, Yu Zhao, Chengdan Lai, Hao Li, Mengqi Chen, Longquan Quan, Yunpan Li, Yu Jiang, Wenqi Jia, Lixin Fu, Md Abdul Mazid, Yanling Zhu, Patrick H Maxwell, Guangjin Pan*, Miguel A Esteban*, Zhen Dai*, Liangxue Lai*, Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation, Cell Stem Cell, 2023, 30(9):1235-1245.
3. Sen Yan#*, Xiao Zheng, Yingqi Lin, Caijuan Li, Zhaoming Liu, Jiawei Li, Zhuchi Tu, Yu Zhao, Chunhui Huang, Yizhi Chen, Jun Li, Xichen Song, Bofeng Han, Wei Wang, Weien Liang, Liangxue Lai*, Xiao-Jiang Li*, Shihua Li*, Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington's disease, Nature Biomedical Engineering, 2023, 7(5):629-646.
4. Qin Jin#, Xiaoyi Liu#, Zhenpeng Zhuang#, Jiayuan Huang#, Shixue Gou, Hui Shi, Yu Zhao, Zhen Ouyang, Zhaoming Liu, Lei Li, Junjie Mao, Weikai Ge, Fangbing Chen, Manya Yu, Yezhi Guan, Yinghua Ye, Chengcheng Tang, Ren Huang, Kepin Wang*, Liangxue Lai*, Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation, Genome Biology, 2023, 24:8.
5. Qin Jin#, Xiaoyu Yang, Shixue Gou, Xiaoyi Liu, Zhenpeng Zhuang, Yanhui Liang, Hui Shi, Jiayuan Huang, Han Wu, Yu Zhao, Zhen Ouyang, Quanjun Zhang, Zhaoming Liu, Fangbing Chen, Weikai Ge, Jingke Xie, Nan Li, Chengdan Lai, Xiaozhu Zhao, Jiaowei Wang, Meng Lian, Lei Li, Longquan Quan, Yinghua Ye, Liangxue Lai*, Kepin Wang*, Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression, Science China Life Sciences, 2022, 65(11):2269-2286.
6. Yanhui Liang#, Jingke Xie#, Quanjun Zhang#, Xiaomin Wang#, Shixue Gou, Lihui Lin, Tao Chen, Weikai Ge, Zhenpeng Zhuang, Meng Lian, Fangbing Chen, Nan Li, Zhen Ouyang, Chengdan Lai, Xiaoyi Liu, Lei Li, Yinghua Ye, Han Wu, Kepin Wang*, Liangxue Lai*, AGBE: A dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns, Nucleic Acids Research, 2022, 50(9):5384-5399.
7. Xiaomin Wang#, Yanhui Liang#, Jianping Zhao#, Yuan Li, Shixue Gou, Min Zheng, Juanjuan Zhou, Quanjun Zhang, Jidong Mi*, Liangxue Lai*, Generation of permanent neonatal diabetes mellitus dogs with glucokinase point mutations through base editing, Cell Discovery, 2021, 7:92.
8. Jingke Xie#, Weikai Ge#, Nan Li#, Qishuai Liu#, Fangbing Chen, Xiaoyu Yang, Xingyun Huang, Zhen Ouyang, Quanjun Zhang, Yu Zhao, Zhaoming Liu, Shixue Gou, Han Wu, Chengdan Lai, Nana Fan, Qin Jin, Hui Shi, Yanhui Liang, Ting Lan, Longquan Quan, Xiaoping Li, Kepin Wang*, Liangxue Lai*, Efficient base editing for multiple genes/loci in pigs using base editors, Nature Communications, 2019, 10:2852.
9. Sen Yan#, Zhuchi Tu#, Zhaoming Liu#, Nana Fan, Huiming Yang, Su Yang, Weili Yang, Yu Zhao, Zhen Ouyang, Chengdan Lai, Huaqiang Yang, Li Li, Qishuai Liu, Hui Shi,2 Guangqing Xu, Heng Zhao, Hongjiang Wei, Zhong Pei, Shihua Li*, Liangxue Lai*, Xiao-Jiang Li*, A huntingtin knock-in pig model recapitulates features of selective neurodegeneration in Huntington’s disease, Cell, 2018, 173(4):989-1002.
10. Kepin Wang#, Qin Jin#, Degong Ruan#, Yi Yang, Qishuai Liu, Han Wu, Zhiwei Zhou, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Bentian Zhao, Quanjun Zhang, Jiangyun Peng, Chengdan Lai, Nana Fan, Yanhui Liang, Ting Lan, Nan Li, Xiaoshan Wang, Xinlu Wang, Yong Fan, Pieter A. Doevendans, Joost P.G. Sluijter, Pentao Liu, Xiaoping Li*, Liangxue Lai*, Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing, Genome Research, 2017, 27(12): 2061-2071.
11.Yi Yang#, Kepin Wang#, Han Wu, Qin Jin, Degong Ruan, Zhen Ouyang, Bentian Zhao, Zhaoming Liu, Yu Zhao, Quanjun Zhang, Nana Fan, Qishuai Liu, Shimei Guo, Lei Bu, Yong Fan, Xiaofang Sun, Xiaoping Li*, Liangxue Lai*, Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering, Journal of Molecular Cell Biology, 2016, 8(2): 174-177.
12. Guohao Wang#, Huaqiang Yang#, Sen Yan#, Chuan-En Wang, Xudong Liu, Bentian Zhao, Zhen Ouyang, Peng Yin, Zhaoming Liu, Yu Zhao, Tao Liu, Nana Fan, Lin Guo, Shihua Li, Xiao-Jiang Li*, Liangxue Lai*, Cytoplasmic mislocalization of RNA splicing factors and aberrant neuronal gene splicing in TDP-43 transgenic pig brain, Molecular Neurodegeneration, 2015, 10:42.
13. Huaqiang Yang#, Guohao Wang#, Haitao Sun#, Runzhe Shu#, Tao Liu, Chuan-En Wang, Zhaoming Liu, Yu Zhao, Bentian Zhao, Zhen Ouyang, Dongshan Yang, Jiao Huang, Yueling Zhou, Shihua Li, Xiaodan Jiang*, Zhicheng Xiao*, Xiao-Jiang Li*, Liangxue Lai*, Species-dependent neuropathology in transgenic SOD1 pigs, Cell Research, 2014, 24(4):464-481.
14. Xiaoping Li#, Yi Yang#, Lei Bu, Xiaogang Guo, Chengcheng Tang, Jun Song, Nana Fan, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Xiaoling Yi, Longquan Quan, Songcai Liu, Zhenguo Yang, Hongsheng Ouyang, Y Eugene Chen, Zhong Wang*, Liangxue Lai*, Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing, Cell Research, 2014, 24(4):501-504.
15. Jun Song, Juan Zhong, Xiaogang Guo, Yongqiang Chen, Qingjian Zou, Jiao Huang, Xiaoping Li, Quanjun Zhang, Zhiwu Jiang, Chengcheng Tang, Huaqiang Yang, Tao Liu, Peng Li, Duanqing Pei, Liangxue Lai, Generation of RAG 1- and 2-deficient rabbits by embryo microinjection of TALENs, Cell Research, 2013, 23(8):1059-1062.
16. Nana Fan#, Jijun Chen#, Zhouchun Shang#, Hongwei Dou, Guangzhen Ji, Qingjian Zou, Lu Wu, Lixiazi He, Fang Wang, Kai Liu, Na Liu, Jianyong Han, Qi Zhou, Dengke Pan, Dongshan Yang, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Lin Lin, Chongming Zhong, Quanlei Wang, Shouqi Wang, Ying Xu, Jing Luan, Yu Liang, Zhenzhen Yang, Jing Li, Chunxia Lu, Gábor Vajta, Ziyi Li, Hongsheng Ouyang, Huayan Wang, Yong Wang, Yang Yang, Zhonghua Liu, Hong Wei, Zhidong Luan, Miguel A Esteban, Hongkui Deng, Huanming Yang, Duanqing Pei, Ning Li, Gang Pei, Lin Liu, Yutao Du*, Lei Xiao*, Liangxue Lai*, Piglets cloned from induced pluripotent stem cells, Cell Research, 2013, 23(1):162-166.
17. Dongshan Yang#, Huaqiang Yang#, Wei Li, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Nana Fan, Jun Song, Jiangtian Tian, Feng Li, Jifeng Zhang, Lin Chang, Duanqing Pei, Y Eugene Chen*, Liangxue Lai*, Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning, Cell Research, 2011, 21(6):979-982.
18. Liangxue Lai#, Jing X Kang#, Rongfeng Li, Jingdong Wang, William T Witt, Hwan Yul Yong, Yanhong Hao, David M Wax, Clifton N Murphy, August Rieke, Melissa Samuel, Michael L Linville, Scott W Korte, Rhobert W Evans, Thomas E Starzl, Randall S Prather*, Yifan Dai*, Generation of cloned transgenic pigs rich in omega-3 fatty acids, Nature Biotechnology, 2006, 24(4):435-436.
19. Donna Kolber-Simonds#, Liangxue Lai#, Steven R Watt#, Maria Denaro#, Scott Arn, Monica L Augenstein, Jeffery Betthauser, David B Carter, Julia L Greenstein, Yanhong Hao, Gi-Sun Im, Zhonghua Liu, Greg D Mell, Clifton N Murphy, Kwang-Wook Park, August Rieke, David J J Ryan, David H Sachs, Erik J Forsberg, Randall S Prather, Robert J Hawley*, Production of alpha-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations, Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(19):7335-7340.
20. Liangxue Lai#, Donna Kolber-Simonds, Kwang-Wook Park, Hee-Tae Cheong, Julia L Greenstein, Gi-Sun Im, Melissa Samuel, Aaron Bonk, August Rieke, Billy N Day, Clifton N Murphy, David B Carter, Robert J Hawley, Randall S Prather*, Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning, Science, 2002, 295(5557):1089-1092.