People
LI Zhiyuan
Title：Group Leader
Subject：
Email：li_zhiyuan@gibh.ac.cn
Address：190 Kaiyuan Avenue, Guangzhou Science Park, Huangpu District, Guangzhou
Study/Work Experience

2008.09 – now

Guangzhou Institutes of Biomedicine and Health (CAS)/ Principal Investigator

2004.07 – 2008.08

Saint Louis University, Faculty of Pharmacological and Physiological Sciences/Assistant Professor

2000.07 – 2004.06

Vanderbilt University, School of Medicine, Pharmacology Department/ Post-doctoral Fellow

1997.09 – 2000.06

Tokushima University, School of Medicine, Nutrition Department/ Ph.D

1994.09 – 1997.06

Central South University, XiangYa school of Medicine, Pharmacology Department/ Ph.D

Research Areas

①   Stem Cell Basic and Clinical Research

②   Ion Channels Properties of Neurons Differentiation from iPSCs

③   P2X Molecular Functional Structure

④   TRPVs Involve in Cancer Regulation

⑤   Drug Scanning and Safety Evaluation by hERK Ion Channels


1. Li Zhiyuan, Cheng Na, Wang Fei, Lin Zuoxian, Ye Wei. A serum-free medium for mesenchymal stem cells. (ZL201610560103.2) 

2. Li Zhiyuan, Wen-bo Ma, Deng Sihao, Li Caiyue, Chen Dan, Liu Liang. A lysate and method for extracting ancient biological skeleton DNA. (ZL201610014145.6) 

3. Li Zhiyuan, Huang Rongqi, Long Yan, Lin Zuoxian, Wen-bo Ma. Application of paeonol in preparation of drugs for activating hERG ion channels. (ZL201510955053.3) 

4. Yin Shikui, Li Caiyue, Li Zhiyuan. A semi-automatic sucker subpackaging instrument (April 20151416924). 

5. Li Zhiyuan, Li Caiyue, Wen-bo Ma. An efficient method for extracting TRPV3 protein from mouse skin (201510290429.3). 

6. Li Zhiyuan, Gao Chao, Liu Jingxin. A intracellular fluid for rapidly increasing cell membrane permeability and its method (ZL201510280313.1). 

7. Li Zhiyuan, Deng Sihao, Xue Zhiqin, He Xu, Zhong Xiaolin, Hu Xing. A culture medium for promoting the differentiation of rat neural stem cells and its application method (ZL201410288590.2). 

8. Li Zhiyuan, Deng Sihao, Cao Wenyu, Lu Dahua, Idy. A cryopreservation solution of neural stem cells and its application method (ZL 20151007248. X).

9. Li Zhiyuan, Hu Jianguang, Cao Wenyu, Deng Sihao, Lu Dahua, Idy. A corpse specimen preservation solution (ZL201510037247.5). 

10. Li Zhiyuan, Zhou Yuye, Xiao Zhuo. Neural stem cell differentiation medium and its application (ZL2015101146927). 

11. Li Zhiyuan, Liang Xin, Yin Shikui. A method that can quickly increase the permeability of cell membrane and thus quickly break the membrane to form a high-resistance seal (ZL201310473138.9). 

12. Li Zhiyuan, Jie Yanling, Xu Huijuan, Liang Xin. Amines, their preparation methods and their application in the preparation of anti-influenza drugs (ZL201310585676.7). 

13. Li Zhiyuan, Cheng Na, Chen Jiaqi, Liang Dongquan. Separator (magnetic ring plate) (ZL201330030300.0) 

14. Li Zhiyuan, Cheng Na, Chen Jiaqi, Liang Dongquan. Separator (magnetic bar plate) (ZL201330030240.2) 

15. Li Zhiyuan, Cheng Na, Chen Jiaqi, Liang Dongquan. A microporous plate magnetic bead separator (ZL201320057660.4). 

16. Li Zhiyuan, Cheng Na, Chen Jiaqi, Liang Dongquan. A micro magnetic bead separator (ZL2013 2 0057672.7). 

17. Li Zhiyuan, Yin Shikui, Jie Yanling. A plasmid extraction and adsorption column (ZL201320179011.1). 

18. Li Zhiyuan, Jie Yanling, Yin Shikui, Xu Huijuan. An improved seismic platform (ZL2013 2 0497741.6). 

19. Li Zhiyuan, Yin Shikui, Li Caiyue, A new guide column antibody binding collection tube in Wen-bo Ma (ZL201320497668.2). 

20. Li Zhiyuan, Xu Huijuan, Jie Yanling, Liang Xin: An improved cell cryopreservation box (ZL201320627288.6).



Academic Performance

Li Zhiyuan; Huang Jufang; Deng sihao; Liu Jingxin; Wen-bo Ma; Hu Xing. Study on ion channel-related small molecular compounds regulating the fate of stem cells, natural science award, third prize, 2018.

Representative Papers

1.  Basnet R, Basnet BB, Amissah OM, HuangR, SunY, HabimanaJDD,LiZ.          (2024) Network pharmacology-based virtual screening of chemical constituents from Vitexnegundo Linn’s discovered novel molecular targets for breast cancer treatment. Curr. Cancer Drug Targets. Online ahead of print.

2.  Basnet R, Amissah OM, Basnet BB, Huang R, Sun Y, Habimana JDD, Li Z. (2024) Potential target of CDK6 signaling pathway for cancer treatment perspective. Curr. Drug Targets. Online ahead of print.

3.   Wang L, Liang Z, Guo Y, Habimana JdD, Ren Y, Amissah OB, Mukama O, Peng S, Ding X, Lv L, Li J, Chen M, Liu Z, Huang R, Zhang Y, Li Y, Li Z*, Sun Y. (2024) STING agonist diABZI enhances the cytotoxicity of T cell towards cancer cells. Cell Death & Disease. 15(4).

4.  Amissah, OB, Chen W, de Dieu Habimana J, Sun Y, Lin L, Liu Y, Wang L, Liu Z, Mukama O, Basnet R, Liu H, Li J, Ding X, Lv L, Chen M, Liang Y, Huang R, Li Z. (2024) NY-ESO-1-specific T cell receptor-engineered T cells and Tranilast, a TRPV2 antagonist bivalent treatment enhances the killing of esophageal cancer: a dual-targeted cancer therapeutic route. Cancer Cell Int. 24(1):64.

5.  Amissah OB, Basnet R, Chen W, Habimana JdD, Baiden BE, Owusu OA, Saeed BJ, Li Z. (2024) Enhancing antitumor response by efficiently generating large-scale TCR-T cells targeting a single epitope across multiple cancer antigens. Cellular Immunology. 399-400:104827.

6.  Peng K, Xie W, Wang T, Li Y, Habimana JDD, Amissah OB, Huang J, Chen Y, Ni B, Li Z. (2023) HIF-1α promotes kidney organoid vascularization and applications in disease modeling. Stem cell Research & Therapy. 19;14(1):336

7.  Song A, Wang W, Zhang Y, Zhou P, Li J, Habimana JDD, Mukama O, Xie W, Deng S, Zhang S, Li M, Ni B, Tang Y, Yan X, Huang J, Li Z. (2023) Acetylsalicylic Acid Promotes Osteogenic Differentiation of Human Dental Pulp Mesenchymal Stem Cells and Regeneration of Alveolar Bone in Experimental Periodontitis Rats. Journal of Tissue Engineering and Regenerative Medicine. Volume 2023, Article ID 3077814, 13 pages

8.  de Dieu Habimana, J., Mukama, O., Amissah, O. B., Sun, Y., Karangwa, E., Liu, Y., Mugisha, S., Cheng, N., Wang, L., Chen, J., Deng, S., Huang, R., & Li, Z. (2023). A Rationally Designed CRISPR/Cas12a Assay Using a Multimodal Reporter for Various Readouts. Analytical chemistry, 95(31), 11741–11750.

9.  Liu Y, Amissah OB, Huangfang X, Wang L, Dieu Habimana J, Lv L, Ding X, Li J, Chen M, Zhu J, Mukama O, Sun Y, Li Z, Huang R（2023）. Large-scale expansion of human umbilical cord-derived mesenchymal stem cells using PLGA@PLL scaffold. Bioresour Bioprocess. 10(1):18.

10.  Xie W, Shi L, Quan H, Xiao H, Chen J, Liu J, de Dieu Habimana J, Huang R, Luo J, Chen P, Li Z (2023). SYVN1 ubiquitinates FoxO1 to induce β-catenin nuclear translocation, PD-L1-mediated metastasis, and immune evasion of hepatocellular carcinoma. Cell Oncol (Dordr).  Epub ahead of print.

11.  Habimana JD, Mukama O, Chen G, Chen M, Amissah OB, Wang L, Liu Y, Sun Y, Li AL, Deng S, Huang J, Yan XX, Rutaganda T, Mutangana D, Wu LP, Huang R, Li Z(2022). Harnessing enhanced CRISPR/Cas12a trans-cleavage activity with extended reporters and reductants for early diagnosis of Helicobacter pylori, the causative agent of peptic ulcers and stomach cancer. Biosens Bioelectron. 222:114939.

12.  Huang R, Li S, Tian C, Zhou P, Zhao F, Xie W, Xiao J, Wang L, Habimana JDD, Lin Z, Yang Y, Cheng N, Li Z. (2022) Thermal stress involved in TRPV2 promotes tumorigenesis through the pathways of HSP70/27 and PI3K/Akt/mTOR in esophageal squamous cell carcinoma. British Journal of Cancer. 127(8) ,1424-1439.

13.  Han X, Habimana JDD, Li AL, Huang R, Mukama O, Deng W, Zhang Y, Wang W, Deng S, Peng K, Ni B, Zhang S, Huang J, Yan X, Li Z. (2022) Transcription factor EB-mediated mesenchymal stem cell therapy induces autophagy and alleviates spinocerebellar ataxia type 3 defects in neuronal cells model. Cell Death Diseases. 13(7):622.

14.  Zhang Y, Deng W, Wang W, Song A, Mukama O, Deng S, Han X, Habimana JDD, Peng K, Ni B, Zhang S, Huang J, Yan X, Li Z. (2022) MicroRNA-206 Down-regulated Human Umbilical Cord Mesenchymal Stem Cells Alleviate Cognitive Decline in D-galactose-induced Aging Mice. Cell Death Discovery. Jul 4;8(1):304.

15.  Habimana JD, Huang R, Muhoza B, Kalisa YN, Han X, Deng W, Li Z. (2022) Mechanistic insights of CRISPR/Cas nucleases for programmable targeting and early-stage diagnosis. Biosens Bioelectron. 25;203:114033.

16.  Mukunzi D, Habimana JDD, Li Z, Zou X. (2022) Mycotoxins detection: view in the lens of molecularly imprinted polymer and nanoparticles. Crit Rev Food sci Nutr. Jan 20;1-35.

17.  Yi Z, Habimana JDD, Mukama O, Li Z, Odiwuor N, Jing H, Nie C, Hu M, Lin Z, Wei H, and Zeng L. (2022) Rational Programming of Cas12a for Early-Stage Detection of COVID-19 by Lateral Flow Assay and Portable Real-time Fluorescence Readout Facilities, Biosensors.2(1):11.

18.  Zhao H, Li S, Lin Z, He L, Deng W, Han X, Zhang Y, Ni B, Tang F, Cheng N, Zhou P, Huang R, Deng S, Huang J, Li Z. (2022). Ameliorating Effect of Umbilical Cord Mesenchymal Stem Cells in a Human Induced Pluripotent Stem Cell Model of Dravet Syndrome. Molecular Neurobiology. 59(2):748-761.

19.  Deng W, Zhang Y, Wang W, Song A, Mukama O, Huang J, Han X, Deng S, Lin Z, Habimana J.D., Huang R, Peng K, Ni B, Zhang S, Yan X, Li J, Wu L, Li Z. (2021) Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle. Stem cell Research & Therapy. 12(1):548 .

20.  Long Y, Hou J, Tang F, Lin Z, Huang X, Li W, Chen Y, Li Z, Wu Z. (2021). Proarrhythmic effects induced by benzethonium chloride and domiphen bromide in vitro and in vivo. (2021) Toxicology and Applied Pharmacology 431 (2021) 115731.

21.  He L, Wang S, Peng L, Zhao H, Li S, Han X, Habimana J.D., Chen Z, Wang C, Peng Y, Peng H, Xie Y, Lijing Lei L, Deng Q, Wan L, Wan N, Yuan H, Gong Y, Zou G, Li Z, Tang B & Jiang H. (2021). CRISPR/Cas9 mediated gene correction ameliorates abnormal phenotypes in spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cells. Translational Psychiatry. 11:479

22.  Deng S, Zhang Y, Liao Z, Huang J, Huang R, Li Z. (2021). S100A4 plays a key role in TRPV3 ion channel expression and its electrophysiological function. Neurosci. Lett. 759:135999.

23. He L, Han X, Zhao H, Li S, Chen Z, Wang C, Wang S, Peng L, Deng W, Li Z, Tang B, Jiang H. (2021)Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line (CSUXHi005-A) from human urine epithelial cells. Stem Cell Res. 53:102289.

24.  Liao Z, Wang W, Deng W, Zhang Y, Song A, Deng S, Zhao H, Zhang S, Li Z. (2021) Human umbilical cord mesenchymal stem cells-secreted TSG-6 is anti-inflammatory and promote tissue repair after spinal cord injury. ASN Neuro. 13: 17590914211010628.

25.  Li S; Zhao H; Han X; Ni B; He L; Mukama O; Habimana JDD; Lin Z; Huang R; Huang H; Tian C; Tang F; Li Z. (2021) Generation of UCiPSCs-derived neurospheres for cell therapy and its application. Stem Cell Research & Therapy. 12(1):188.

26.  Liao Z, Yang X, Wang W, Deng W, Zhang Y, Song A, Ni B, Zhao H, Zhang S, Li Z. (2021) hucMSCs transplantation promotes locomotor function recovery, reduces apoptosis and inhibits demyelination after SCI in rats. Neuropeptides. 86:102125.

27.  Zhao H, Li S, Lin Z, He L, Deng W, Han X, Tang F, Cheng N, Zhou P, Huang R, Deng S, Huang J, Li Z. (2020). A heterozygous SCN1A (c.A5768G/+) mutant human induced pluripotent stem cell line (USTCi002-A) generated using TALEN-mediated precise gene editing. Stem Cell Res. 49, 102058.

28.  Zhao H, He L, Huang H, Li S, Cheng N, Tang F, Han X, Lin Z, Huang R, Zhou P, Deng S, Huang J, Li Z. (2020). Generation of a tdTomato-GAD67 reporter human epilepsia mutation induced pluripotent stem cell line, USTCi001-A-2, using CRISPR/Cas9 editing. Stem Cell Res. 48:102003.

29.  Zhou P, Xie W, Huang HL, Huang R, Tian C, Zhu H, Dai Y, Li Z. (2020). circRNA_100859 functions as an oncogene in colon cancer by sponging the miR-217-HIF-1α pathway. Aging (Albany NY). 12(13):13338-13353.

30.  Cheng N, Liu Y, Mukama O , Han X, Huang H, Li S, Zhou P, Lu X, Li Z. (2020). A signal-enhanced and sensitive lateral flow aptasensor for the rapid detection of PDGF-BB. RSC Adv. 10, 18601.

31.  Zhao H, He L, Li S, Huang H, Tang F, Han X, Lin Z, Tian C, Huang R, Zhou P, Huang J, Deng S, Li Z. (2020). Generation of corrected-hiPSC (USTCi001-A-1) from epilepsy patient iPSCs using TALEN-mediated editing of the SCN1A gene. Stem Cell Res. 46:101864.

32.  Tian C, Han X, He L, Tang F, Huang R, Lin Z, Li S, Deng S, Xu J, Huang H, Zhao H, Li Z. (2020). Transient receptor potential ankyrin 1 contributes to the ATP-elicited oxidative stress and inflammation in THP-1-derived macrophage. Molecular and cellular biochemistry, 473(1-2):179-192.

33.  Tian C, Li S, He L, Han X, Tang F, Huang R, Lin Z, Deng S, Xu J, Huang H, Zhao H, Li Z.(2020).Transient receptor potential ankyrin 1 contributes to the lysophosphatidylcholine-induced oxidative stress and cytotoxicity in OLN-93 oligodendrocyte. Cell Stress Chaperones. 25(6):955-968.

34.  Zhao H, Li S, He L, Han X, Huang H, Tang F, Lin Z, Deng S, Tian C, Huang R, Li Z. (2020). Generation of iPSC line (USTCi001-A) from human skin fibroblasts of a patient with epilepsy. Stem Cell Res. 45:101785.

35.  Tian C, Huang R, Tang F, Lin Z, Cheng N, Han X, Li S, Zhou P, Deng S, Huang H, Zhao H, Xu J, Li Z. (2020). Transient Receptor Potential Ankyrin 1 Contributes to Lysophosphatidylcholine-Induced Intracellular Calcium Regulation and THP-1-Derived Macrophage Activation. J Membr Biol. 253(1):43-55.

36.  He L, Zhao H, Li S, Han X, Chen Z, Wang C , Tian C, Tang F, Huang R, Lin Z, Li Z, Tang B, Jiang H.(2020).Generation of Induced Pluripotent Stem Cell Line (CSUXHi002-A) From a Patient With Spinocerebellar Ataxia Type 1. Stem Cell Res. 45:101816.

37.  Mukama O, Yuan T, He Z, Li Z, Habimana J, Hussain M, Li W, Yi Z, Liang Q, Zeng L.(2020).A high fidelity CRISPR/Cas12a based lateral flow biosensor for the detection of HPV16 and HPV18. Sensors and Actuators B: Chemical. 316: 128119.

38.  Mukama O, Wu j, Li Z, Liang Q, Yi Z, Lu X, Liu Y, Liu Y, Hussain M, Makafe G, Liu J, Xu N, Zeng L.(2020).An ultrasensitive and specific point-of-care CRISPR/Cas12 based lateral flow biosensor for the rapid detection of nucleic acids. Biosensors and Bioelectronics. 159: 112143.

39.  Yuan T , Mukama O , Li Z , Chen W , Zhang Y , de Dieu Habimana J , Zhang Y , Zeng R , Nie C , He Z , Zeng L . (2020).  A rapid and sensitive CRISPR/Cas12a based lateral flow biosensor for the detection of Epstein-Barr virus. Analyst. 28;145(19):6388-6394.

40.  Wu J, Mukama O, Wu W, Li Z, Habimana JD, Zhang Y, Zeng R, Nie C, Zeng L. (2020). A CRISPR/Cas12a Based Universal Lateral Flow Biosensor for the Sensitive and Specific Detection of African Swine-Fever Viruses in Whole Blood. Biosensors, 10(12), 203.