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INSTITUTION AND LOCATION |
DEGREE (if applicable) |
MM/YY |
FIELD OF STUDY |
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Nanjing University, China |
B.S. |
07/01 |
Biophysics |
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Tsinghua University, China |
Ph.D. |
07/06 |
Structural Biology |
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Oxford University, UK |
Visitor |
05/06 |
Cryo-Electron Microscopy |
|
National University of Singapore, Singapore |
Visitor |
03/07 |
Biological Electron Microscopy (EMBO Course) |
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The Scripps Research Institute, CA, USA |
Visitor |
08/08 |
Cryo-Electron Microscopy |
|
Jul. 2006 – present |
Professor, Principal Investigator; Laboratory of Biological Electron Microscopy and Structural Biology (Fei Sun’s lab), Institute of Biophysics, Chinese Academy of Sciences |
|
Jul. 2006 – present |
Director and Chief Scientist; Center for Biological Imaging, Core Facilities for Protein Sciences, Institute of Biophysics, Chinese Academy of Sciences |
|
Sep. 2015 – present |
Professor; School of Life Science, University of Chinese Academy of Sciences |
|
Jun. 2019 – present |
Adjunct Professor; BioLand Laboratory, Guangzhou, Guangdong Province, China |
Development of high-throughput and high-resolution cell ultrastructure lineage atlas technology and equipment based on dual-modality imaging using fluorescence microscopy and electron microscopes.
Cell ultrastructure lineage atlas technology and equipment development
Cell ultrastructure lineage atlas technology links the cell lineage with the physical connection of cells within the tissue, establishing a three-dimensional cell lineage atlas with spatial resolution, and on this basis, outlines its dynamic changes, thereby obtaining information about cell evolution, organ development, disease occurrence, and aging processes, effectively promoting biomedical progress.
We aim to develop cell ultrastructure lineage atlas technology and equipment. Based on self-developed high-throughput Transmission Electron Microscopy and Fluorescence Microscopy, we integrate various technical processes of cell ultrastructure lineage analysis through optoelectronic correlation fluorescence probes and automated software, achieving automatic serial ultrathin sections of cell tissue samples, Fluorescence Microscopic imaging, high-throughput Transmission Electron Microscopy imaging with fluorescence navigation, and dual-modality ultrastructural analysis of cell tissue samples, ultimately establishing a complete high-throughput high-resolution Optoelectronic Correlation Imaging solution.
Zhu Li Yuehua Outstanding Teacher Award, CAS, 2022
First Prize of Beijing Science and Technology Progress Award, 2021
China National Funds for Distinguished Young Scientists, 2020
National Middle-&-Youth Talent of Science and Technology Innovation, 2019
Youth professor, Chang Jiang Scholars Program of China, 2018
Outstanding contribution of Chinese cryo-electron microscopy society, 2017
National Youth Top-notch Talent, 2012
Beishi Zhang Prize for Young Scientist in Biophysics, 2009
Top 100, Excellent Ph.D thesis of China, 2008
1. Zhai Yujia; Sun Fei. SmartBM-1, an expression shuttle vector in insect cell-mammalian cell and its application. ZL202311601025.2.
2. Zhu Boling; Ye Zhinan; Chen Yongsheng; Huang Xiaojun; Zhu Yun; Sun Fei. A preparation system and preparation method for inactivated Cryo-EM Samples. 20231095P2698.6.
3. He Jia; Sun Wenhao; Zhang Yan; Sun Fei; Yang Ge. Isotropic reconstruction of large-scale biological electron microscopy based on self-supervised deep learning technique. ZL202211689462.X.
4. Liu Guole; Niu Tongxin; Qiu Mengxuan; Sun Fei; Yang Ge; Zhu Yun. Particle picking methods, devices and electronic equipment for cryo-electron microscopy based on deep learning. ZL202211284170.8.
5. Ji Gang; Huang Xiaojun; Sun Fei. A preparation method of ultra-thin microarray support film for transmission electron microscopy. ZL201910843744.2.
6. Ji Gang; Li Xixia, Zhang Jianguo, Sun Fei. An automatic focusing method for scanning/ transmission electron microscopy imaging. ZL201910034869.0.
7. Fan Hongcheng; Sun Fei; Qiao Mingqiang; Wang Bo; Zhu Yun. A preparation method of electron microscope grid using hydrophobin membrane as supporting membrane. ZL202110576212.4.
8. Zhai Yujia; Sun Fei. A method and special system for expressing n proteins or protein subunits simultaneously. ZL 201610248592.8.
9. Zhai Yujia; Sun Fei. SmartBac Baculovirus Expression System and Its Application. ZL 201810028508.0.
10. Sun Fei; Pang Xiaoyun; Zhai Yujia; Wang Ganggang; Xie Tian. DNA molecule for presenting target protein on bacteria surface and its application. ZL201310750736.6.
11. Huang Xiaojun; Ji Gang; Sun Fei. Preparation method of grid of transmission electron microscope. ZL201510355339.8.
12. Ji Gang; Li Shuoguo; Sun Fei. An optical vacuum cooling stage for correlation imaging of light and electron microscopy. ZL201410363314.8.
13. Ji Gang; Wang Li; Xu Wei; Sun Fei. Shaking device. ZL201410550483.2.
1）Scientific research
1. Xu, J.#, Liao, C.#, Yin, C.C., Li, G.*, Zhu, Y.*, and Sun, F.* (2024). In situ structural insights into the excitation-contraction coupling mechanism of skeletal muscle. Sci Adv 10, eadl1126.
2. Kong, W.#, Zhu, Y.#, Zhao, H., Du, K., Zhou, R., Li, B., Yang, F., Hou, P., Huang, X., Chen, Y., Wang, Y., Sun, F.*, Jiang, Y.* & Zhou, C.* (2024). Cryo-electron tomography reveals the packaging pattern of RuBisCOs in Synechococcus beta-carboxysome. Structure. 32, 1110-1120 e4.
3. Zhai, C., Zhang, N., Li, X., Tan, X., Sun, F.*, Dong, M.* (2024). Immuno-electron microscopy localizes Caenorhabditis elegans vitellogenins along the classic exocytosis route. Life Metabolism, 3(6), loae025.
4. Zheng, L.#, Yang, T.#, Guo, H.#*, Qi, C.#, Lu, Y. #, Xiao, H., Gao, Y., Liu, Y., Yang, Y., Zhou, M., Nguyen, H., Zhu, Y.*, Sun, F.*, Zhang, C.*, Ji, X.* (2024). Cryo-EM structures of human SID-1 transmembrane family proteins and implications for their low-pH-dependent RNA transport activity. Cell Research 34, 80-83.
5. Wen, Z., Zhang, Y., Zhang, B., Hang, Y., Xu, L., Chen, Y., Xie, Q., Zhao, Q., Zhang, L., Li, G., Zhao, B., Sun, F.*, Zhai, Y.*, Zhu, Y.*, et al. (2023). Cryo-EM structure of the cytosolic AhR complex. Structure 31, 295-308 e294.
6. Tai, L.#, Yin, G.#, Huang, X., Sun, F.*, and Zhu, Y.* (2023). In-cell structural insight into the stability of sperm microtubule doublet. Cell Discov 9, 116.
7. Zhao, M.#, Zhu, Y.#, Zhang, L.#, Zhong, G.#, Tai, L., Liu, S., Yin, G., Lu, J., He, Q., Li, M., Zhao, R., Wang, H., Huang, W., Fan, C., Shuai, L., Wen, Z., Wang, C., He, X., Chen, Q., Liu, B., Xiong, X., Bu, Z.*, Wang, Y.*, Sun, F.*, Yang, J.* (2022). Novel cleavage sites identified in SARS-CoV-2 spike protein reveal mechanism for cathepsin L-facilitated viral infection and treatment strategies. Cell Discov 8, 53.
8. Zhai, C., Zhang, N., Li, X., Chen, X., Sun, F.*, and Dong, M.* (2022). Fusion and expansion of vitellogenin vesicles during Caenorhabditis elegans intestinal senescence. Aging Cell, e13719.
9. Tai, L.#, Zhu, Y.#, Ren, H.#, Huang, X., Zhang, C.*, and Sun, F.* (2022). 8 A structure of the outer rings of the Xenopus laevis nuclear pore complex obtained by cryo-EM and AI. Protein Cell 13, 760-777.
10. Zhu, G.#, Zeng, H.#*, Zhang, S.#, Juli, J., Tai, L., Zhang, D., Pang, X., Zhang, Y., Lam, S.M., Zhu, Y.*, Peng, G.*, Michel, H.*, Sun, F.* (2021). The Unusual Homodimer of a Heme-Copper Terminal Oxidase Allows Itself to Utilize Two Electron Donors. Angew Chem Int Ed Engl 60, 13323-13330.
11. Zhang, Y.#, Pang, X.#*, Li, J., Xu, J., Hsu, V.W.*, and Sun, F.* (2021). Structural insights into membrane remodeling by SNX1. Proc Natl Acad Sci U S A 118.
12. Xia, S.#, Lan, Q.#, Zhu, Y.#, Wang, C.#, Xu, W.#, Li, Y., Wang, L., Jiao, F., Zhou, J., Hua, C., Wang, Q., Cai, X., Wu, Y., Gao, J., Liu, H., Sun, G., Munch, J., Kirchhoff, F., Yuan, Z., Xie, Y., Sun, F.*, Jiang, S.*, Lu, L.* (2021). Structural and functional basis for pan-CoV fusion inhibitors against SARS-CoV-2 and its variants with preclinical evaluation. Signal Transduct Target Ther 6, 288.
13. Tai, L.#, Zhu, G.#, Yang, M.#, Cao, L., Xing, X., Yin, G., Chan, C., Qin, C., Rao, Z., Wang, X.*, Sun, F.*, Zhu, Y.* (2021). Nanometer-resolution in situ structure of the SARS-CoV-2 postfusion spike protein. Proc Natl Acad Sci U S A 118.
14. Du, J.#, Wang, D.#, Fan, H.#, Xu, C.#, Tai, L.#, Lin, S.#, Han, S., Tan, Q., Wang, X., Xu, T., Zhang, H., Chu, X., Yi, C., Liu, P., Wang, X., Zhou, Y., Pin, J., Rondard, P., Liu, H.*, Liu, J.*, Sun, F.*, Wu, B.*, Zhao, Q.* (2021). Structures of human mGlu2 and mGlu7 homo- and heterodimers. Nature 594, 589-593.
15. Zhu, G.#, Zeng, H.#, Zhang, S.#, Juli, J., Pang, X., Hoffmann, J., Zhang, Y., Morgner, N., Zhu, Y.*, Peng, G.*, Michel, H.*, Sun, F.* (2020). A 3.3 A-Resolution Structure of Hyperthermophilic Respiratory Complex III Reveals the Mechanism of Its Thermal Stability. Angew Chem Int Ed Engl 59, 343-351.
16. Zhang, D.#, Zhang, Y.#, Ma, J., Zhu, C., Niu, T., Chen, W., Pang, X., Zhai, Y., and Sun, F.* (2020). Cryo-EM structures of S-OPA1 reveal its interactions with membrane and changes upon nucleotide binding. Elife 9: e50294.
17. Shi, Y.#, Xin, Y.#, Wang, C.#, Blankenship, R.E., Sun, F.*, and Xu, X.* (2020). Cryo-EM structures of the air-oxidized and dithionite-reduced photosynthetic alternative complex III from Roseiflexus castenholzii. Sci Adv 6, eaba2739.
18. Qiao, A.#, Han, S.#, Li, X.#, Li, Z.#, Zhao, P.#, Dai, A., Chang, R., Tai, L., Tan, Q., Chu, X., Ma, L., Thorsen,T., Runge, S., Yang, D., Wang, M., Sexton, P., Wootten, D.*, Sun, F.*, Zhao, Q.*, Wu, B.* (2020). Structural basis of Gs and Gi recognition by the human glucagon receptor. Science 367, 1346-1352.
19. Lu J.#, Chan C.#, Yu L.#, Fan J.*, Sun F.* and Zhai Y.* (2020) Molecular mechanism of mitochondrial phosphatidate transfer by Ups1. Communications Biology, 3: 468.
20. Xia S.#, Liu M.#, Wang C.#, Xu W.#, Lan Q., Feng S., Qi F., Bao L., Du L., Liu S., Qin C., Sun F.&, Shi Z.&, Zhu Y.*&, Jiang S.*&, and Lu L.*& (2020) Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion. Cell Research, 30 (4): 343-355. (&: co-senior authors)
21. Ren, Z.#, Zhang, Y.#, Zhang, Y., He, Y., Du, P., Wang, Z., Sun, F.*, and Ren, H.* (2019). Cryo-EM Structure of Actin Filaments from Zea mays Pollen. Plant Cell 31, 2855-2867.
22. Gong H.#, LI L.#, Xu A.#, Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen H.Y., Lam S.M., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson C., Wong L.L., Guddat L.W., Sun F.*, Wang Q.* and Rao Z.* (2018), An electron transfer path connects subunits of a mycobacterial respiratory supercomplex. Science 362.
23. Xin Y.#, Shi Y.#, Niu T.#, Wang Q., Niu W., Huang X., Ding W., Yang L., Blankenship R. E., Xu X.* and Sun F.* (2018) Cryo-EM structure of the RC-LH core complex from an early branching photosynthetic prokaryote. Nature communications, 9:1568.
24. Lu G.#, Xu Y.#, Zhang K., Xiong Y., Li H., Cui L., Wang X., Lou J., Zhai Y.*, Sun F.* and Zhang X.C.* (2017), Crystal structure of E. coli apolipoprotein N-acyltransferase. Nature Communications, 8:15948.
25. Chen R.#, Gao B.#, Liu, X., Ruan F., Zhang Y., Lou J., Feng K., Wunsch C., Li S.M., Dai J.* and Sun F.* (2017), Molecular insights into the enzyme promiscuity of an aromatic prenyltransferase. Nat Chem Biol 13, 226-234.
26. Wei R.#, Wang X.#, Zhang Y., Mukherjee S., Zhang L., Chen Q., Huang X., Jing S., Liu C., Li S., Wang G., Xu Y., Zhu S., Williams A., Sun F.* and Yin C.C.* (2016), Structural insights into Ca2+ -activated long-range allosteric channel gating of RyR1. Cell Research 26: 977-994 (Cover story).
27. Zhou, Q.#, Li, J.#, Yu, H., Zhai, Y., Gao, Z., Liu, Y., Pang, X., Zhang, L., Schulten, K., Sun, F.*, Chen, C.* (2014). Molecular insights into the membrane-associated phosphatidylinositol 4-kinase IIalpha. Nature communications 5, 3552.
28. Pang, X., Fan, J., Zhang, Y., Zhang, K., Gao, B., Ma, J., Li, J., Deng, Y., Zhou, Q., Egelman, E.H., Hsu, V.W.*, Sun, F.* (2014). A PH domain in ACAP1 possesses key features of the BAR domain in promoting membrane curvature. Developmental cell 31, 73-86.
29. Wang, X.#, Xu, F.#, Liu, J.#, Gao, B., Liu, Y., Zhai, Y., Ma, J., Zhang, K., Baker, T.S., Schulten, K., Zheng, D.*, Pang, H.*, Sun, F.* (2013). Atomic model of rabbit hemorrhagic disease virus by cryo-electron microscopy and crystallography. PLoS Pathog 9, e1003132.
2）Technology development
1. Liu, G.#, Niu, T.#, Qiu, M., Zhu, Y., Sun, F.*, and Yang, G*. (2024). DeepETPicker: Fast and accurate 3D particle picking for cryo-electron tomography using weakly supervised deep learning. Nature communications 15, 2090.
2. Li, S.#, Wang, Z.#, Jia, X., Niu, T., Zhang, J., Yin, G., Zhang, X., Zhu, Y.*, Ji, G.*, and Sun, F.* (2023). ELI trifocal microscope: a precise system to prepare target cryo-lamellae for in situ cryo-ET study. Nat Methods 20, 276-283.
3. Li, S., Jia, X., Niu, T., Zhang, X., Qi, C., Xu, W., Deng, H., Sun, F.*, and Ji, G.* (2023). HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography. Commun Biol 6, 474.
4. Li, X.#, Lazic, I.# *, Huang, X.#, Wirix, M., Wang, L., Deng, Y., Niu, T., Wu, D., Yu, L., and Sun, F.* (2022). Imaging biological samples by integrated differential phase contrast (iDPC) STEM technique. J Struct Biol 214, 107837.
5. Zhang, J.#, Zhang, D.#, Sun, L.#, Ji, G., Huang, X., Niu, T., Xu, J., Ma, C., Zhu, Y., Gao, N., Xu, W., Sun, F.* (2021). VHUT-cryo-FIB, a method to fabricate frozen hydrated lamellae from tissue specimens for in situ cryo-electron tomography. J Struct Biol 213, 107763.
6. Huang, X.#, Zhang, L.#, Wen, Z., Chen, H., Li, S., Ji, G., Yin, C.C., and Sun, F.* (2021). Amorphous nickel titanium alloy film: A new choice for cryo electron microscopy sample preparation. Prog Biophys Mol Biol 160, 5-15.
7. Fan, H., Wang, B., Zhang, Y., Zhu, Y., Song, B., Xu, H., Zhai, Y., Qiao, M.*, and Sun, F.* (2021). A cryo-electron microscopy support film formed by 2D crystals of hydrophobin HFBI. Nature communications 12, 7257.
8. Zhai, Y.*, Zhang, D., Yu, L., Sun, F., and Sun, F.* (2019). SmartBac, a new baculovirus system for large protein complex production. J Struct Biol X 1, 100003.
9. Li X.#, Zhang S.#, Zhang J. and Sun F.* (2018), In situ protein micro-crystal fabrication by cryo-FIB for electron diffraction. Biophysics Reports, 4(6): 339-347. doi: 10.1007/s41048-018-0075-x.
10. Li S., Ji G.*, Shi Y., Klausen L.H., Niu T., Wang S., Huang X., Ding W., Zhang X., Dong M., Xu W., and Sun F.* (2018), High-vacuum optical platform for cryo-CLEM(HOPE): a new solution for non-integrated multiscale correlative light and electron microscopy. Journal of Structural Biology, 201(1): 63-75.
11. Wang, S., Li, S., Ji, G., Huang, X., and Sun, F.* (2017). Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ. Biophys Rep 3, 8-16.
12. Shi, Y., Wang, L., Zhang, J., Zhai, Y., and Sun, F.* (2017). Determining the target protein localization in 3D using the combination of FIB-SEM and APEX2. Biophys Rep 3, 92-99.
13. Li, X.#, Ji, G.#*, Chen, X., Ding, W., Sun, L., Xu, W., Han, H., and Sun, F.* (2017). Large scale three-dimensional reconstruction of an entire Caenorhabditis elegans larva using AutoCUTS-SEM. J Struct Biol 200, 87-96.
14. Han, R.#, Wan, X.#, Wang, Z., Hao, Y., Zhang, J., Chen, Y., Gao, X., Liu, Z., Ren, F., Sun, F.*, Zhang, F.* (2017). AuTom: A novel automatic platform for electron tomography reconstruction. J Struct Biol 199, 196-208.
15. Zhang, J.#, Ji, G.#, Huang, X., Xu, W.*, and Sun, F.* (2016). An improved cryo-FIB method for fabrication of frozen hydrated lamella. J Struct Biol 194, 218-223.
16. Shan, H., Wang, Z., Zhang, F., Xiong, Y., Yin, C.C.*, and Sun, F.* (2016). A local-optimization refinement algorithm in single particle analysis for macromolecular complex with multiple rigid modules. Protein Cell 7, 46-62.
17. Deng, Y.#, Chen, Y.#, Zhang, Y., Wang, S., Zhang, F.*, and Sun, F.* (2016). ICON: 3D reconstruction with 'missing-information' restoration in biological electron tomography. J Struct Biol 195, 100-112.
18. Chen, Y.#, Zhang, Y.#, Zhang, K., Deng, Y., Wang, S., Zhang, F.*, and Sun, F.* (2016). FIRT: Filtered iterative reconstruction technique with information restoration. J Struct Biol 195, 49-61.
19. Han, R., Wang, L., Liu, Z., Sun, F.*, and Zhang, F.* (2015). A novel fully automatic scheme for fiducial marker-based alignment in electron tomography. J Struct Biol 192, 403-417.